Journal: Neurobiology of disease

Article Title: An animal model of Miller Fisher Syndrome: mitochondrial hydrogen peroxide is produced by the autoimmune attack of nerve terminals and activates Schwann cells

doi: 10.1016/j.nbd.2016.09.005

Figure Lengend Snippet: Anti-GQ1b plus complement induces ERK1/2 phosphorylation in SCs co-cultured with neurons. (A) Representative Western blot showing ERK phosphorylation induced by 30 min incubation with FS3+NHS in SCs co-cultured with SCMNs. Phospho-ERK levels are reduced upon pre-incubation with catalase. No ERK activation is detected following exposure to NHS or when NHS is heat-inactivated. FS3+NHS fails to increase p-ERK levels in isolated SCMNs. (B) Quantification of p-ERK levels normalized on Hsp90 signal. N=5, Student’s unpaired t-test, two-sides, *p<0.05, ***p<0.001. (C) Phospho-ERK signal (green) is restricted to SCs in neurons-SCs co-cultures treated with FS3+NHS for 30 min, in line with Western blot results. Neurons are detectable by FS3 staining (red). Nuclei are stained by Hoechst (blue). Scale bar: 10 μm.

Article Snippet: For densitometric quantification, the bands of interest were normalized to the housekeeping protein Hsp90 (mouse monoclonal, 1:1000, BD Transduction Laboratories).

Techniques: Cell Culture, Western Blot, Incubation, Activation Assay, Isolation, Staining