Journal: Neurotoxicology

Article Title: Microarray genomic profile of mitochondrial and oxidant response in Manganese Chloride treated PC12 cells

doi: 10.1016/j.neuro.2012.01.001

Figure Lengend Snippet: HO-1 expression in MnCl2 treated PC12 cells after 24 Hrs incubation. Verification of altered gene expression of array was performed by RT-PCR. HO-1 band intensities were measured using Molecular Dynamics 300 Series Computing Densitometer (4A), normalized against corresponding RPS29 housekeeping bands (4B). The data represent the Mean ± S.E.M , n=3 and difference from the control was analyzed using a students t-test. * p<.05.

Article Snippet: Film images were scanned and protein band intensities were quantitated using Molecular Dynamics 300 Series Computing Densitometer .

Techniques: Expressing, Incubation, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TNF-α and IL-10 Control CXCL13 Expression in Human Macrophages.

doi: 10.4049/jimmunol.1900790

Figure Lengend Snippet: FIGURE 2. CXCL13 expression is induced in activated AM and MoDM and is controlled by NF-kB. AM and MoDM from patients with IPF (A and B) and MoDM from healthy donors (C–G) were cultured without (M0) or with 20 ng/ml LPS for 24 h or other indicated times. In some experiments, MoDMs were first treated with 5 mM Bay (C–E) or transiently transfected with control, RelB, or p100/p52 Si-RNA for 24 h (F and G). Relative CXCL13 mRNA was measured by quantitative RT-PCR and normalized to endogenous ribosomal 18S RNA levels. Data are expressed relative to mRNA levels found in cells stimulated with LPS (A and B), in M0 cells (C), or in cells transfected with control Si-RNA (SiC) and stimulated with LPS (G), arbitrarily set at 1. CXCL13 concentrations in cultured media were measured by ELISA. In (E) and (F), cells were lysed, and protein expression was analyzed by Western blotting. Protein expression was quantified by analyzing each visualized band by densitometry. The results are the means 6 SD of seven (A), 11 (B), four (C, left panel), five (C, right panel), four (D), four (E), and seven (G) independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001 versus LPS (A and B), M0 (C), LPS alone (0) (D and E), and SiC LPS (G).

Article Snippet: Protein expression was quantified by analyzing each visualized band by densitometry (Image Lab Software for total protein normalization; Bio-Rad Laboratories).

Techniques: Expressing, Cell Culture, Transfection, Control, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TNF-α and IL-10 Control CXCL13 Expression in Human Macrophages.

doi: 10.4049/jimmunol.1900790

Figure Lengend Snippet: FIGURE 3. CXCL13 expression is mediated by TNF-a and IL-10 in LPS-stimulated MoDM. MoDMs were untreated (M0, 0) or treated with 5 mM BAY or 2 mg/ml aT or aI and then stimulated with 20 ng/ml LPS for 1 h (C), 24 h (D–G), or the indicated times (A and B). Relative mRNA levels were determined by quantitative RT-PCR and normalized to endogenous ribosomal 18S mRNA levels. Data are expressed relative to mRNA levels found in M0 cells (A) or cells stimulated with LPS alone (0) (C, D, and F), arbitrarily set at 1. Concentrations of TNF-a, IL-10, and CXCL13 secreted in the culture media were quantified by ELISA. In (E), cells were lysed, and protein expression was analyzed by Western blotting. Protein levels were quantified by analyzing each visualized band by densitometry. The results are expressed as the means 6 SD of seven (A), five (B), three (C), three (D, left panel), seven (D, right panel), four (E), seven (F), and five (G) independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001 versus M0 (A and B) and 0 LPS (C–G). #p , 0.05.

Article Snippet: Protein expression was quantified by analyzing each visualized band by densitometry (Image Lab Software for total protein normalization; Bio-Rad Laboratories).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: TNF-α and IL-10 Control CXCL13 Expression in Human Macrophages.

doi: 10.4049/jimmunol.1900790

Figure Lengend Snippet: FIGURE 4. IL-10 enhances CXCL13 expression induced by TNF-a through the JAK/STAT pathway. MoDMs were untreated (M0) or stimulated with 20 ng/ml TNF-a (T) and/or IL-10 (I) for 24 h or with 20 ng/ml LPS for 24 h. In (B), cells were lysed, and protein expression was analyzed by Western blotting. Protein levels were quantified by analyzing each visualized band by densitometry. Relative mRNA levels were determined by quantitative RT-PCR and normalized to endogenous ribosomal 18S RNA levels. Data are expressed relative to mRNA levels found in M0 cells (A and C) or in cells stimulated with LPS alone (0) (F and G), arbitrarily set at 1. Concentrations of TNF-a, IL-10, and CXCL13 secreted in the culture media were quantified by ELISA. The results are expressed as the means 6 SD of six (A, left panel), four (A, right panel), seven (B), nine (C), six (D), four (E), and five (F and G) independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001 versus M0 (A–D) or 0 LPS (E–G). #p , 0.05.

Article Snippet: Protein expression was quantified by analyzing each visualized band by densitometry (Image Lab Software for total protein normalization; Bio-Rad Laboratories).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay